Its name is derived from the Greek words χλωρός, khloros ("pale green") and φύλλον, phyllon ("leaf").
Conversely, it is a poor absorber of green and near-green portions of the spectrum, which it reflects, producing the green color of chlorophyll-containing tissues.
The presence of magnesium in chlorophyll was discovered in 1906, and was that element's first detection in living tissue.
In 1967, the last remaining stereochemical elucidation was completed by Ian Fleming, and in 1990 Woodward and co-authors published an updated synthesis.
Chlorophyll f was announced to be present in cyanobacteria and other oxygenic microorganisms that form stromatolites in 2010; a molecular formula of C55H70O6N4Mg and a structure of (2-formyl)-chlorophyll a were deduced based on NMR, optical and mass spectra.
In these complexes, chlorophyll serves three functions.
The function of the vast majority of chlorophyll (up to several hundred molecules per photosystem) is to absorb light.
Having done so, these same centers execute their second function: the transfer of that light energy by resonance energy transfer to a specific chlorophyll pair in the reaction center of the photosystems.
This pair effects the final function of chlorophylls, charge separation, leading to biosynthesis.
These centres are named after the wavelength (in nanometers) of their red-peak absorption maximum.
The identity, function and spectral properties of the types of chlorophyll in each photosystem are distinct and determined by each other and the protein structure surrounding them.
The function of the reaction center of chlorophyll is to absorb light energy and transfer it to other parts of the photosystem.
The absorbed energy of the photon is transferred to an electron in a process called charge separation.
The removal of the electron from the chlorophyll is an oxidation reaction.
The chlorophyll donates the high energy electron to a series of molecular intermediates called an electron transport chain.
The charged reaction center of chlorophyll (P680) is then reduced back to its ground state by accepting an electron stripped from water.
The electron that reduces P680 ultimately comes from the oxidation of water into O2 and H through several intermediates.
This reaction is how photosynthetic organisms such as plants produce O2 gas, and is the source for practically all the O2 in Earth's atmosphere.
Photosystem I typically works in series with Photosystem II; thus the P700 of Photosystem I is usually reduced as it accepts the electron, via many intermediates in the thylakoid membrane, by electrons coming, ultimately, from Photosystem II.
Electron transfer reactions in the thylakoid membranes are complex, however, and the source of electrons used to reduce P700 can vary.
The electron flow produced by the reaction center chlorophyll pigments is used to pump H ions across the thylakoid membrane, setting up a chemiosmotic potential used mainly in the production of ATP (stored chemical energy) or to reduce NADP to NADPH.
NADPH is a universal agent used to reduce CO2 into sugars as well as other biosynthetic reactions.
Reaction center chlorophyll–protein complexes are capable of directly absorbing light and performing charge separation events without the assistance of other chlorophyll pigments, but the probability of that happening under a given light intensity is small.
Thus, the other chlorophylls in the photosystem and antenna pigment proteins all cooperatively absorb and funnel light energy to the reaction center.
Besides chlorophyll a, there are other pigments, called accessory pigments, which occur in these pigment–protein antenna complexes.
Chlorophylls are numerous in types, but all are defined by the presence of a fifth ring beyond the four pyrrole-like rings.
They share a common biosynthetic pathway with porphyrins, including the precursor uroporphyrinogen III.
For the structures depicted in this article, some of the ligands attached to the Mg center are omitted for clarity.
The chlorin ring can have various side chains, usually including a long phytol chain.
The most widely distributed form in terrestrial plants is chlorophyll a.
The structures of chlorophylls are summarized below:
|Chlorophyll a||Chlorophyll b||Chlorophyll c1||Chlorophyll c2||Chlorophyll d||Chlorophyll f|
|Occurrence||Universal||Mostly plants||Various algae||Various algae||Cyanobacteria||Cyanobacteria|
- Structures of chlorophylls
Measurement of chlorophyll content
Measurement of the absorption of light is complicated by the solvent used to extract the chlorophyll from plant material, which affects the values obtained,
- In diethyl ether, chlorophyll a has approximate absorbance maxima of 430 nm and 662 nm, while chlorophyll b has approximate maxima of 453 nm and 642 nm.
- The absorption peaks of chlorophyll a are at 465 nm and 665 nm. Chlorophyll a fluoresces at 673 nm (maximum) and 726 nm. The peak molar absorption coefficient of chlorophyll a exceeds 10 M cm, which is among the highest for small-molecule organic compounds.
- In 90% acetone-water, the peak absorption wavelengths of chlorophyll a are 430 nm and 664 nm; peaks for chlorophyll b are 460 nm and 647 nm; peaks for chlorophyll c1 are 442 nm and 630 nm; peaks for chlorophyll c2 are 444 nm and 630 nm; peaks for chlorophyll d are 401 nm, 455 nm and 696 nm.
By measuring the absorption of light in the red and far red regions, it is possible to estimate the concentration of chlorophyll within a leaf.
Ratio fluorescence emission can be used to measure chlorophyll content.
By exciting chlorophyll a fluorescence at a lower wavelength, the ratio of chlorophyll fluorescence emission at 705±10 nm and 735±10 nm can provide a linear relationship of chlorophyll content when compared with chemical testing.
The ratio F735/F700 provided a correlation value of r 0.96 compared with chemical testing in the range from 41 mg m up to 675 mg m. Gitelson also developed a formula for direct readout of chlorophyll content in mg m. The formula provided a reliable method of measuring chlorophyll content from 41 mg m up to 675 mg m with a correlation r value of 0.95.
Main article: Chlorophyllide
Chlorophyll b is made by the same enzyme acting on chlorophyllide b.
Chlorophyll itself is bound to proteins and can transfer the absorbed energy in the required direction.
Hence, plants need an efficient mechanism of regulating the amount of this chlorophyll precursor.
In angiosperms, this is done at the step of aminolevulinic acid (ALA), one of the intermediate compounds in the biosynthesis pathway.
Plants that are fed by ALA accumulate high and toxic levels of protochlorophyllide; so do the mutants with a damaged regulatory system.
Senescence and the chlorophyll cycle
The process of plant senescence involves the degradation of chlorophyll: for example the enzyme chlorophyllase (EC ) hydrolyses the phytyl sidechain to reverse the reaction in which chlorophylls are biosynthesised from chlorophyllide a or b.
Since chlorophyllide a can be converted to chlorophyllide b and the latter can be re-esterified to chlorophyll b, these processes allow cycling between chlorophylls a and b.
Moreover, chlorophyll b can be directly reduced (via 7-hydroxychlorophyll a) back to chlorophyll a, completing the cycle.
In later stages of senescence, chlorophyllides are converted to a group of colourless tetrapyrroles known as nonfluorescent chlorophyll catabolites (NCC's) with the general structure:
Defective environments can cause chlorosis
Further information: Chlorosis
Soil pH sometimes plays a role in nutrient-caused chlorosis; many plants are adapted to grow in soils with specific pH levels and their ability to absorb nutrients from the soil can be dependent on this.
Chlorosis can also be caused by pathogens including viruses, bacteria and fungal infections, or sap-sucking insects.
Complementary light absorbance of anthocyanins
It may protect the leaves from attacks by plant eaters that may be attracted by green color.
The chlorophyll maps show milligrams of chlorophyll per cubic meter of seawater each month.
Places where chlorophyll amounts were very low, indicating very low numbers of phytoplankton, are blue.
Places where chlorophyll concentrations were high, meaning many phytoplankton were growing, are yellow.
The observations come from the Moderate Resolution Imaging Spectroradiometer (MODIS) on NASA's Aqua satellite.
Land is dark gray, and places where MODIS could not collect data because of sea ice, polar darkness, or clouds are light gray.
The highest chlorophyll concentrations, where tiny surface-dwelling ocean plants are thriving, are in cold polar waters or in places where ocean currents bring cold water to the surface, such as around the equator and along the shores of continents.
It is not the cold water itself that stimulates the phytoplankton.
Instead, the cool temperatures are often a sign that the water has welled up to the surface from deeper in the ocean, carrying nutrients that have built up over time.
In polar waters, nutrients accumulate in surface waters during the dark winter months when plants cannot grow.
When sunlight returns in the spring and summer, the plants flourish in high concentrations.
Chefs use chlorophyll to color a variety of foods and beverages green, such as pasta and spirits.
Absinthe gains its green color naturally from the chlorophyll introduced through the large variety of herbs used in its production.
A 2002 study found that "leaves exposed to strong light contained degraded major antenna proteins, unlike those kept in the dark, which is consistent with studies on the illumination of isolated proteins".
- Bacteriochlorophyll, related compounds in phototrophic bacteria
- Chlorophyllin, a semi-synthetic derivative of chlorophyll
- Deep chlorophyll maximum
- Grow light, a lamp that promotes photosynthesis
- Chlorophyll fluorescence, to measure plant stress
Credits to the contents of this page go to the authors of the corresponding Wikipedia page: en.wikipedia.org/wiki/Chlorophyll.